CNRS, Sorbonne Université Cell-cycle-dependent mRNA localization in P-bodies.
Molecular Cell, 84, 4191-4208.e7 https://doi.org/10.1016/j.molcel.2024.09.011
Safieddine*, A., Benassy, M.-N., Bonte, T., Slimani, F., Pourcelot, O., Kress, M., Ernoult-Lange, M., Courel, M., Coleno, E., Imbert, A., Laine, A., Godebert, A.M., Vinit, A., Blugeon, C., Chevreux, G., Gautheret, D., Walter, T., Bertrand, E., Bénard, M., Weil*, D.
Cv
Adham Safieddine a obtenu un Master en Génomique et Santé de l'Université Libanaise de Beyrouth. Puis il a réalisé sa thèse en biologie moléculaire (Université de Montpellier) dans le laboratoire d'Edouard Bertrand à Montpellier où il a étudié la traduction localisée des ARNm. Il est actuellement post-doctorant au CNRS dans le laboratoire de Dominique Weil (Sorbonne Université) où il s'intéresse aux p-bodies (PBs), des condensats cytoplasmiques d'ARN et de protéines, présents de manière constitutive dans les cellules de mammifères.
Contact
Cette adresse e-mail est protégée contre les robots spammeurs. Vous devez activer le JavaScript pour la visualiser.
Résumé de l'article
Understanding the dynamics of RNA targeting to membraneless organelles is essential to disentangle their functions. Here, we investigate how P-bodies (PBs) evolve during cell-cycle progression in HEK293 cells. PB purification across the cell cycle uncovers widespread changes in their RNA content, partly uncoupled from cell-cycle-dependent changes in RNA expression. Single-molecule fluorescence in situ hybridization (FISH) shows various mRNA localization patterns in PBs peaking in G1, S, or G2, with examples illustrating the timely capture of mRNAs in PBs when their encoded protein becomes dispensable. Rather than directly reflecting absence of translation, cyclic mRNA localization in PBs can be controlled by RBPs, such as HuR in G2, and by RNA features. Indeed, while PB mRNAs are AU rich at all cell-cycle phases, they are specifically longer in G1, possibly related to post-mitotic PB reassembly. Altogether, our study supports a model where PBs are more than a default location for excess untranslated mRNAs.
