Société Française de Biochimie et Biologie Moléculaire
Articles of the month
National Institutes of Health, Bethesda MD 20892, USA"The S. cerevisiae m6A reader Pho92 promotes timely meiotic recombination by controlling key methylated transcripts."
Nucleic Acids Research, 51:517-535 https://doi.org/10.1093/nar/gkac640Jérémy Scutenaire, Damien Plassard, Mélody Matelot, Tommaso Villa, Julie Zumsteg, Domenico Libri, Bertrand Séraphin
PSTEUR INSTITUTE, PARIS CITÉ UNIVERSITY, Structural Microbiology Unity "Interaction network among de novo purine nucleotide biosynthesis enzymes in Escherichia coli."
FEBS J. doi: 10.1111/febs.16746.
Gedeon A, Karimova G, Ayoub N, Dairou J, Giai Gianetto Q, Vichier-Guerre S, Vidalain PO, Ladant D, Munier-Lehmann H.
Institut de Recherches Cliniques de Montréal (IRCM)
"A rationalized definition of general tumor suppressor microRNAs excluded miR-34a"
Nucleic Acid Res. 50, 4703-4712 DOI:10.1093/nar/gkac277
Mockly S., Houbron E. & Seitz H.
CNRS, Université Paris Cité"Sen1 is a key regulator of transcription-driven conflicts."Molecular Cell. DOI:10.1016/ j.molcel.2022.06.021Aiello U, Challal D, Wentzinger G, Lengronne A, Appanah R, Pasero P, Palancade B, Libri D. (2022)
EMBL, Grenoble "Structural basis for sequence-independent substracte selection by eukaryotic wobble base tRNA deaminase ADAT2/3"
Nature Communication2022, 13, 6737 https://doi.org/10.1038/s41467-022-34441-z
Dolce L.G., Zimmer A.A., Tengo L., Weis F., Rubio M.A.T., Alfonzo J.D. & Kowalinski E.
Cv
Luciano Dolce is a 31 year-old structural biologist. He is interested in RNA editing and modification, with a focus on the protein complexes that perform and regulate them. He did his PhD in Brazil, at the LNBio - Laboratório Nacional de Biociências, applying crystallography and biophysical techniques to study protein-protein complexes. After his PhD, he moved to Grenoble to work as a postdoctoral fellow in Eva Kowalinski's group at EMBL, where he uses Cryo-electron microscopy to better understand RNA editing and modifications in the parasite Trypanosoma brucei.
Contact
Luciano DOLCE
71 avenue des Martyrs
38000 Grenoble
twitter @LgDolce
Résumé de l'article
The essential deamination of adenosine A34 to inosine at the wobble base is the individual tRNA modification with the greatest effects on mRNA decoding, empowering a single tRNA to translate three different codons. To date, many aspects of how eukaryotic deaminases specifically select their multiple substrates remain unclear. Here, using cryo-EM, we present the structure of a eukaryotic ADAT2/3 deaminase bound to a full-length tRNA, revealing that the enzyme distorts the anticodon loop, but in contrast to the bacterial enzymes, selects its substrate via sequence-independent contacts of eukaryote-acquired flexible or intrinsically unfolded motifs distal from the conserved catalytic core. A gating mechanism for substrate entry to the active site is identified. Our multi-step tRNA recognition model yields insights into how RNA editing by A34 deamination evolved, shaped the genetic code, and directly impacts the eukaryotic proteome.
Cell Structural Biology (BIOC),Polytechnique School "The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex." iScience, 25: 104646. DOI: 10.1016/j.isci.2022.104646
Limei Zou, Clara Moch, Marc Graille & Clément Chapat
PASTEUR INSTITUTE
"The genomic landscape of contemporary western Remote Oceanians."
Current Biology. https://doi.org/10.1016/J.CUB.2022.08.055
Arauna, L. R., Bergstedt, J., Choin, J., Mendoza-Revilla, J., Harmant, C., Roux, M., Mas-Sandoval, A., Lémée, L., Colleran, H., François, A., Valentin, F., Cassar, O., Gessain, A., Quintana-Murci, L., & Patin, E.
Paris ENS - Chemical Department "RNA at the surface of phase-separated condensates impacts their size and number"
Biophysical Journal, 121, Pages 1675-1690. doi.org/10.1016/j.bpj.2022.03.032
Audrey Cochard, Marina Garcia-Jove Navarro, Leonard Piroska, Shunnichi Kashida, Michel Kress, Dominique Weil, Zoher Gueroui
Laboratory of Cell Structural Biology, BIOC, Polytechnique School, CNRS."Role of aIF5B in archaeal translation initiation."
Nucleic Acids Research, 50, 6532–6548, https://doi.org/10.1093/nar/gkac490
Kazan R., Bourgeois G., Lazennec-Schurdevin C., Larquet E., Mechulam Y., Coureux P.-D., Schmitt E.
Laboratory INFINITE - MEDICINE FACULTY OF LILLE “A porous cervical mucus plug leads to preterm birth induced by experimental vaginal infection in mice”
iScience , 10.1016/j.isci.2022.104526
Guillaume Lacroix; Valérie Gouyer; Mylène Rocher; Frédéric Gottrand; Jean-Luc Desseyn (2022)
