Société Française de Biochimie et Biologie Moléculaire


IBPC, CNRS UMR8261 "Identification and characterization of RNA binding sites for (p)ppGpp using RNA-DRaCALA."
Nucleic Acids Research/, 10.1093/nar/gkac1224

Jagodnik,J., Tjaden,B., Ross,W. and Gourse,R.L.

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Dept. of Molecular Biology and Genetics, Aarhus University, Denmark
« Structures and mechanism of the plant PIN-FORMED auxin transporter »
Nature 609, 605–610 (2022) https://doi.org/10.1038/s41586-022-04883-y
Kien Lam Ung*, Mikael Winkler*, Lukas Schulz, Martina Kolb, Dorina P. Janacek, Emil Dedic, David L. Stokes, Ulrich Z. Hammes & Bjørn Panyella Pedersen.

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National Institutes of Health, Bethesda MD 20892, USA"The S. cerevisiae m6A reader Pho92 promotes timely meiotic recombination by controlling key methylated transcripts."
Nucleic Acids Research, 51:517-535 https://doi.org/10.1093/nar/gkac640Jérémy Scutenaire, Damien Plassard, Mélody Matelot, Tommaso Villa, Julie Zumsteg, Domenico Libri, Bertrand Séraphin

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PSTEUR INSTITUTE, PARIS CITÉ UNIVERSITY, Structural Microbiology Unity "Interaction network among de novo purine nucleotide biosynthesis enzymes in Escherichia coli." 
FEBS J.  doi: 10.1111/febs.16746.
Gedeon A, Karimova G, Ayoub N, Dairou J, Giai Gianetto Q, Vichier-Guerre S, Vidalain PO, Ladant D, Munier-Lehmann H.

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Institut de Recherches Cliniques de Montréal (IRCM)
"A rationalized definition of general tumor suppressor microRNAs excluded miR-34a" 
Nucleic Acid Res. 50, 4703-4712 DOI:10.1093/nar/gkac277

Mockly S., Houbron E. & Seitz H.

 

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CNRS, Université Paris Cité"Sen1 is a key regulator of transcription-driven conflicts."Molecular Cell. DOI:10.1016/ j.molcel.2022.06.021Aiello U, Challal D, Wentzinger G, Lengronne A, Appanah R, Pasero P, Palancade B, Libri D. (2022

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EMBL, Grenoble  "Structural basis for sequence-independent substracte selection by eukaryotic wobble base tRNA deaminase ADAT2/3"

Nature Communication2022, 13, 6737 https://doi.org/10.1038/s41467-022-34441-z
Dolce L.G., Zimmer A.A., Tengo L., Weis F., Rubio M.A.T., Alfonzo J.D. & Kowalinski E.


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Luciano Dolce is a 31 year-old structural biologist. He is interested in RNA editing and modification, with a focus on the protein complexes that perform and regulate them. He did his PhD in Brazil, at the LNBio - Laboratório Nacional de Biociências, applying crystallography and biophysical techniques to study protein-protein complexes. After his PhD, he moved to Grenoble to work as a postdoctoral fellow in Eva Kowalinski's group at EMBL, where he uses Cryo-electron microscopy to better understand RNA editing and modifications in the parasite Trypanosoma brucei.

Contact

Luciano DOLCE

71 avenue des Martyrs

38000 Grenoble

twitter @LgDolce

Résumé de l'article

The essential deamination of adenosine A34 to inosine at the wobble base is the individual tRNA modification with the greatest effects on mRNA decoding, empowering a single tRNA to translate three different codons. To date, many aspects of how eukaryotic deaminases specifically select their multiple substrates remain unclear. Here, using cryo-EM, we present the structure of a eukaryotic ADAT2/3 deaminase bound to a full-length tRNA, revealing that the enzyme distorts the anticodon loop, but in contrast to the bacterial enzymes, selects its substrate via sequence-independent contacts of eukaryote-acquired flexible or intrinsically unfolded motifs distal from the conserved catalytic core. A gating mechanism for substrate entry to the active site is identified. Our multi-step tRNA recognition model yields insights into how RNA editing by A34 deamination evolved, shaped the genetic code, and directly impacts the eukaryotic proteome.

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Cell Structural Biology (BIOC),Polytechnique School "The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex." iScience, 25: 104646. DOI: 10.1016/j.isci.2022.104646
Limei Zou, Clara Moch, Marc Graille & Clément Chapat

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PASTEUR INSTITUTE

"The genomic landscape of contemporary western Remote Oceanians."

Current Biology. https://doi.org/10.1016/J.CUB.2022.08.055
Arauna, L. R., Bergstedt, J., Choin, J., Mendoza-Revilla, J., Harmant, C., Roux, M., Mas-Sandoval, A., Lémée, L., Colleran, H., François, A., Valentin, F., Cassar, O., Gessain, A., Quintana-Murci, L., & Patin, E.

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Paris ENS -  Chemical Department "RNA at the surface of phase-separated condensates impacts their size and number"
Biophysical Journal, 121, Pages 1675-1690. doi.org/10.1016/j.bpj.2022.03.032
Audrey Cochard, Marina Garcia-Jove Navarro, Leonard Piroska, Shunnichi Kashida, Michel Kress, Dominique Weil, Zoher Gueroui

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