CIRI - U1111 INSERM - UMR 5308 CNRS - UCBL - ENSL
“Proteomic analysis of the host- pathogen interface in experimental cholera.”
Nat Chem Biol. 2021 Nov 17. doi.org/10.1101/2021.01.05.425471
Zoued A, Zhang H, Zhang T, Giorgio RT, Kuehl CJ, Fakoya B, Sit B, Waldor MK
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Abdelrahim Zoued completed his undergraduate studies in Marseille at the Aix-Marseille University. He then obtained a fellowship to carry out his Ph.D. in Eric Cascales’lab in 2011. The key goals of his Ph.D. were to elucidate the biogenesis of a prevalent type of bacterial secretion system, the Type 6 Secretion System. He then joined the lab of Pr. Matthew Waldor at Harvard Medical School in Boston for his postdoctoral studies. His principal objective was to gain new insights into changes in the bacterial and host cell surface proteome during infection and deepen our understanding of cholera pathogenesis. In this paper, he and his collaborator used biochemical and proteomic approaches to establish a new approach to reveal the proteins present at the host-V. cholerae interface during infection. He found that SP-D, a host C-type lectin that directly binds the V. cholerae surface, is a novel intestinal defense factor. Abdelrahim Zoued recently joined the lab of Patricia Doublet at the CIRI in Lyon to study the pathogenic bacterium Legionella pneumophila.
CONTACT:
Abdelrahim Zoued Ph.D.
CIRI - Centre International de Recherche en Infectiologie U1111 INSERM - UMR 5308 CNRS - UCBL - ENSL
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Résumé de l'article
The microbial cell surface is a site of critical microbe–host interactions that often control infection outcomes. Defining the set of host proteins present at this interface has been challenging. Here we used a surface-biotinylation approach coupled to quantitative mass spectrometry to identify and quantify both bacterial and host proteins present on the surface of diarrheal fluid-derived Vibrio cholerae in an infant rabbit model of cholera. The V. cholerae surface was coated with numerous host pro- teins, whose abundance were driven by the presence of cholera toxin, including the C-type lectin SP-D. Mice lacking SP-D had enhanced V. cholerae intestinal colonization, and SP-D production shaped both host and pathogen transcriptomes. Additional host proteins (AnxA1, LPO and ZAG) that bound V. cholerae were also found to recognize distinct taxa of the murine intestinal microbiota, suggesting that these host factors may play roles in intestinal homeostasis in addition to host defense.
