Institute of Molecular and Cellular Biology CNRS, Institute of Genetics and of Molecular and Cellular Biology CNRS, Strasbourg University, Strasbourg, France Structure of the 70S ribosome from human pathogen Staphylococcus aureusNucleic Acids Research, 2016, Vol. 44, No. 21 10491–10504 Iskander Khusainov†, Quentin Vicens†, Anthony Bochler, François Grosse, Alexander Myasnikov, Jean-François Ménétret, Johana Chicher, Stefano Marzi, Pascale Romby, Gulnara Yusupova, Marat Yusupov and Yaser Hashem.
†Co-first authors
Cv
Iskander Khusainov, 28 ans, effectue actuellement un post-doctorat dans le laboratoire de Marat Yusupov à l’IGBMC (Illkirch). Après des études à l'Université fédérale de Kazan en Russie (2006-2011), il a rejoint l'Université de Strasbourg, où il a réalisé une thèse soutenue en 2015. Ses travaux de recherche ont pour fil conducteur les bactéries pathogènes. Il s’est d’abord intéressé aux mécanismes moléculaires des voies réglementaires de détection de quorum chez la bactérie pathogène des plantes Erwinia carotovora, ainsi qu’à la réponse au stress et la production de facteurs de virulence. Il étudie dorénavant la bactérie pathogène humaine Staphylococcus aureus et en particulier les mécanismes de synthèse des protéines de cette bactérie. Ses travaux visent à caractériser les éléments structuraux de leur ribosome, responsables de la résistance aux antibiotiques utilisés cliniquement.
Contact
This email address is being protected from spambots. You need JavaScript enabled to view it.
Team Marat Yusupov
IGBMC, 1 rue Laurent Fries
67404, Illkirch Cedex
+3388653353
Abstract
Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely related organisms, their existence could account for vital yet species-specific mechanisms of translation regulation that would involve stalling, cell survival and antibiotic resistance. Here, we present the first full 70S ribosome structure from Staphylococcus aureus, a Gram-positive pathogenic bacterium, solved by cryo-electron microscopy. Comparative analysis with other known bacterial ribosomes pinpoints several unique features specific to S. aureus around a conserved core, at both the protein and the RNA levels. Our work provides the structural basis for the many studies aiming at understanding translation regulation in S. aureus and for designing drugs against this often multi-resistant pathogen.
