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PhD position in structural biology of IDP and protein-membrane interaction by NMR spectroscopy

IECB / CBMN

ANR-funded PhD position in structural biology of IDP and protein-membrane interaction by NMR spectroscopy

 

Project: This ANR-funded project is focused on exploring the role and the assembly mechanisms of plant-specific proteins called REMORINs (REMs). REMORINs are crucial hub proteins involved in plant defense against viral propagation by regulating cell-to-cell connectivity and are tightly associated in the assembly of nanodomains at the plant plasma membrane (PM). The different group of REMs label distinct domains of the PM. They possess a N-terminal domain that is intrinsically disordered (IDP) and a C-terminal domain interaction with specific anionic lipids at the PM. REMORIN contain phosphorylation sites and the exact biological function of phosphorylation and its impact on the structural architecture and protein-lipid interaction remain unexplored.

Here we aim at investigating the structural and dynamic organization of REMORIN, in its full-length form as well as in the context of the isolated IDP and the C-terminal membrane-bound domain. Solid-state and solution Nuclear Magnetic Resonance (NMR) will be the main methods to decipher at atomic detail the role of phosphorylation during the protein-membrane targeting. Several partners interacting with the IDP have been identified and we aim at deciphering how phosphorylation and protein-protein interaction will impact the conformational state of REMORIN. In parallel, cell-to-cell connectivity experiments and in planta imaging of fluorescently tagged-proteins will be performed to correlate how phosphorylation-dependent protein-protein interaction is linked to viral response.

 

Laboratory: http://www.loquetlab.org/ Our laboratory is fully equipped for molecular biology, protein expression and purification. We manage the NMR platform of the IECB/CBMN in Bordeaux (France), including 300, 500, 600, 700 and 800MHz NMR spectrometers.

 

Project Partner: The PhD candidate will work at the IECB and will be strongly involved in a collaboration with the team of Sebastien Mongrand, located at the LBM nearby IECB (5km). The candidate will spend a part of the thesis in the Mongrand laboratory to perform confocal microscopy, cell-to-cell connectivity measurements and virus infection in plants

 

Description: The IECB (Bordeaux, France) is an international research center at the interface between biology and chemistry, equipped for structural biology with NMR, mass spectrometry, X-ray crystallography and cryo-EM platforms. Our laboratory “NMR of Molecular Assemblies” is interested in molecular interactions in large biological assemblies and aims at understanding the structure-function interplay. In particular, we develop and apply state-of-the-art solid-state NMR spectroscopy to study atomic architectures and interactions of biological assemblies.

 

Highly motivated candidates should send applications including CV and names of two references to Antoine Loquet (a.loquet@iecb.u-bordeaux.fr) and Birgit Habenstein (b.habenstein@iecb.u-bordeaux.fr). Advantageous expertise includes: interest in structural biology, experience in production and purification of proteins and / or experience in solid-state or solution NMR.

 

Starting date: Available

 

Selected publications of the two groups on REMORINs:

Nanodomain Clustering of the Plant Protein Remorin by Solid-State NMR.

Front Mol Biosci. 2019

 

Mechanisms governing subcompartmentalization of biological membranes.

Curr Opin Plant Biol. 2019

 

REM1.3's phospho-status defines its plasma membrane nanodomain organization and activity in restricting PVX cell-to-cell movement.

PLoS Pathog. 2018

 

Coiled-coil oligomerization controls localization of the plasma membrane REMORINs.

J Struct Biol. 2019

 

Structural basis for plant plasma membrane protein dynamics and organization into functional nanodomains.

Elife. 2017

Publiée le
13.01.2021
Chargé de dossier
Loquet Antoine
Email du contact
Type de contrat
Thèse
Lieu
Bordeaux
Rémunération
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